Evaluation of different methods to discriminate Bacillus anthracis from other bacteria of the Bacillus cereus group
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منابع مشابه
Differentiation of Bacillus anthracis from other Bacillus cereus group bacteria with the PCR.
Variation among isolates of Bacillus anthracis was examined by using restriction fragmentation patterns and the PCR performed with arbitrary and sequence-specific oligonucleotide primers. The patterns were compared with the patterns generated from strains of closely related species belonging to the "Bacillus cereus group" of bacteria, including B. cereus, Bacillus thuringiensis, and Bacillus my...
متن کاملTransduction in Bacillus cereus and Bacillus anthracis.
A phage, designated CP-51, that carries out generalized transduction in Bacillus cereus and B. anthracis, has recently been isolated from soil. Transducing phages for members of the genus Bacillus have been described previously for B. subtilis and B. licheniformis (2-6). Those that have been best characterized are PBS-1 for B. subtilis (4) and SP-10 and SP-15 for both B. subtilis and B. licheni...
متن کاملA Novel Multiplex PCR Discriminates Bacillus anthracis and Its Genetically Related Strains from Other Bacillus cereus Group Species
Anthrax is an important zoonotic disease worldwide that is caused by Bacillus anthracis, a spore-forming pathogenic bacterium. A rapid and sensitive method to detect B. anthracis is important for anthrax risk management and control in animal cases to address public health issues. However, it has recently become difficult to identify B. anthracis by using previously reported molecular-based meth...
متن کاملStrategy for identification of Bacillus cereus and Bacillus thuringiensis strains closely related to Bacillus anthracis.
Bacillus cereus strains that are genetically closely related to B. anthracis can display anthrax-like virulence traits (A. R. Hoffmaster et al., Proc. Natl. Acad. Sci. USA 101:8449-8454, 2004). Hence, approaches that rapidly identify these "near neighbors" are of great interest for the study of B. anthracis virulence mechanisms, as well as to prevent the use of such strains for B. anthracis-bas...
متن کاملGenome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis.
The three species of the group 1 bacilli, Bacillus anthracis, B. cereus, and B. thuringiensis, are genetically very closely related. All inhabit soil habitats but exhibit different phenotypes. B. anthracis is the causative agent of anthrax and is phylogenetically monomorphic, while B. cereus and B. thuringiensis are genetically more diverse. An amplified fragment length polymorphism analysis de...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2006
ISSN: 1364-5072,1365-2672
DOI: 10.1111/j.1365-2672.2006.02809.x